Interplay between the Ña2+-transporting systems of plasma membrane and intracellular Ña2+ stores in the acinar cells of submandibular salivary glands
N. Grychan*, Î. Êîpach**, R. Makarovska***, N. Voitenko**, N. Fedirko*
*Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
**Bogomoletz Institute of Physiology, NASU
4, Bogomoletz St., Kyiv 25001, Ukraine
***Lviv Regional Endocrinologic Dispensary
1, Ostrozky St., Lviv
grychannatali@gmail.com
Regulation of salivation is mediated by the increase of [Ca2+]i in the acinar cells and is regulated by control over spatio-temporal characteristics of [Ca2+]i-signalling. The latter is mediated by coordinated functioning of Ñà2+-transporting systems of plasma membrane (PM) and Ca2+ stores (endoplasmic reticulum (ER) and mitochondria (Mit)) however the mechanisms of this process remain unclear. We showed that activation of the receptor systems in the acinar cells leads to the redistribution of the impact of Ñà2+-sensitive systems in [Ca2+]i-signalling (changes in the activity of Ñà2+-ÀÒPase PM and ER as well as store-operated Ñà2+ entry). Moreover, the mitochondria play a key role in the interplay between the multiple cellular Ñà2+ flows. Thus, described changes represent physiologically relevant adaptation mechanism in which the mitochondria are sensors that coordinate the functioning of Ñà2+-transporting systems in different subcellular organelles and prevent cytotoxic effect of Ñà2+ overload and the ER depletion.
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Keywords: submandibular salivary gland, acinar cells, Ñà2+-homeostasis, plasma membrane, intracellular Ñà2+ store
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