Identification and characterization of streptomyces ghanaensis ATCC 14672 regulatory genes nsdAgh and atrAgh
M. Rabyk*, B. Ostash*, S. Walker**, V. Fedorenko*
*Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
**Harvard Medical School
200, Longwood Àve., Boston, MA 02115, USA
v_fedorenko@franko.lviv.ua
Genes nsdAgh and atrAgh encoding putative regulators of secondary metabolism were identified in the partially sequenced Streptomyces ghanaensis – producer of moenomycins. Both genes were cloned individually into Streptomyces expression vectors and overexpressed in S. ghanaensis and S. coelicolor strains. No significant changes in production of moenomycin A in S. ghanaensis and actinorhodin in S. coelicolor were revealed, implying that the aforementioned genes do not exert significant effects on moenomycin and actinorhodin production under overexpression conditions.
|
Keywords: Streptomyces ghanaensis, moenomycin A, pleiotropic regulators, phylogenetic analysis
|
|