Effects of hydrogen sulfide upon the oxygen uptake and activities of isocitrate and alcohol dehydrogenases in Saccharomyces cerevisiae and Pichia guilliermondii yeasts cells
A. Halushka, S. Gudz
Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
a_halushka@mail.ru
Influence of hydrogen sulfide on the velocity of oxygen uptake by Pichia guilliermondii and Saccharomyces cerevisiae yeasts and on their isocitrate dehydrogenase and alcohol dehydrogenase activities is investigated. Hydrogen sulfide decreases the velocity of oxygen uptake by the cells of S. cerevisiae at concentrations from 10 mM, and by the cells of P. guilliermondii – at concentration 20 mM and higher. Hydrogen sulfide at concentration 30 mM caused the inhibition of P. guilliermondii and S. cerevisiae isocitrate dehydrogenase activity. Concentration of H2S 1 mM increased, and 10–30 mM – decreased the activity of isocitrate dehydrogenase in the cell-free extracts of S. cerevisiae. Hydrogen sulfide stimulated the activity of alcohol dehydrogenase of S. cerevisiae cells. Activity of alcohol dehydrogenase in the cell-free extracts was inhibited at the presence of 10–30 mM of hydrogen sulfide.
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Keywords: hydrogen sulfide, oxygen uptake, isocitrate dehydrogenase, alcohol dehydrogenase, toxicity, P. guilliermondii, S. cerevisiae
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