Variability of metallothioneins in freshwater bivalve Anodonta cygnea (Unionidae) from two feral populations
H. Falfushynska*, L. Gnatyshyna*, Kh. Pryidun*, O. Dedourge-Geffard**, O. Stoliar*, A. Geffard**
*Ternopil National Pedagogical University, 2, Kryvonos St., Ternopil 46027, Ukraine
** Université Reims Champagne Ardenne, Unité Recherche Vignes et Vins de Champagne – Stress Environnement (EA 2069) Laboratoire d\'Eco-Toxicologie
Mou
Oksana.Stolyar@gmail.com
The metallothioneins (MTs) from the digestive gland of the bivalve mollusc Anodonta cygnea were compared in May, July and September at two sites of Dnister River Basin, defined as rural (R) and urbanized (U). The dramatic declining of Cu content in MTs and in the tissue from May till September was accompanied by the appearance of MTs form with molecular weight of about 20 kDa (MT20) in addition to constantly presented MT10 under the size-exclusive chromatography. The chromatography on DEAE-cellulose demonstrated constant MT-1 and variable MT-2/2a forms. The higher levels of Cu or Cd in the MTs reflected their concentrations in the tissue in the R site and the U site correspondingly. The lower metal-binding ability of MTs was reflected at the R site. In spring, the excess of unbound with MTs Zn coupled with the appearance of abundant chromatographic form MT-2a was indicated at the U site.
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Keywords: Freshwater bivalve; Metallothioneins; Feral population
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